Phenotypic Detection of Metallo-β-lactamase among the Clinical Isolates of Imipenem Resistant Pseudomonas and Acinetobacter in Tertiary Care Hospitals of Dhaka City

The rapid spread of Metallo-β-lactamase (MBL) producing Gram negative bacilli represents a matter of great concern worldwide. The study analyzed the occurrence of MBL production in carbapenem resistant Pseudomonas and Acinetobacter isolates over one year period. A total of 132 Pseudomonas and 76 Acinetobacter isolates were obtained from two tertiary care hospitals of Dhaka city. A total of 53 Pseudomonas and 29 Acinetobacter isolates were selected because of their resistance to carbapenem specially imipenem (IPM). Screening for MBL production was performed in these isolates by IPM-EDTA microdilution MIC method. 44 (83%) IPM resistant Pseudomonas and 19 (65.5%) Acinetobacter isolates were MBL producer by IPM-EDTA microdilution MIC method. These results suggest that MBL producing Pseudomonas and Acinetobacter isolates are emerging in our country and it is essential to screen carbapenem resistant isolates for MBL production. Ibrahim Med. Coll. J. 2010; 4(2): 63-65 Ibrahim Med. Coll. J. 2010; 4(2): 63-65 64 Anwar S et al. developed. The microdilution MIC method detects presence of MBL by comparing minimum inhibitory concentration (MICs) of IPM with and without chelators like-EDTA or 2-MPA. In view of the above, the present study was undertaken to determine the prevalence of MBL producing Pseudomonas and Acinetobacter isolates in two tertiary care hospitals of Dhaka city by IPM-EDTA MIC method. Materials and Methods